Methods

This document describes a method for determining the basic efficacy of biocidal active substances in aqueous based fluids and is intended for the generation of data to enable registration of active substances.
Samples of aqueous functional fluids are inoculated with defined suspensions of microorganisms and then incubated. Following the growth of planktonic populations as well as the formation of biofilms on coupons, the samples are treated with the biocidal active substance under test and incubated for a defined period. The changes in the size of the planktonic populations as well as the size of the populations present as biofilms during the post treatment incubation period are used to determine the basic antimicrobial properties of the active substances being evaluated. It is important to note that the results obtained may not describe the performance of the active substance under normal conditions of use.

This document describes a method to determine the resistance of paints and coatings to the growth of Fungi and the effectiveness of biocides (preservatives) that might be used in them. Samples are inoculated with defined suspensions of microorganisms and then incubated. The changes in the size of the populations both over time and in treated compared with untreated materials are used to describe the basic antimicrobial properties of the materials. The results obtained may not describe the performance of the treated materials under normal conditions of use.

This document describes a method for determining the basic efficacy of biocidal active substances in aqueous based paper pulps and is intended for the generation of data to enable registration of active substances. Samples containing the active substance under test are inoculated with defined suspensions of microorganisms and then incubated. The changes in the size of the populations over time are used to determine the basic antimicrobial properties of the active substances being evaluated. It is important to note that the results obtained may not describe the performance of the active substance under normal conditions of use.

This document describes a method for determining the basic efficacy of biocidal active substances in aqueous based Cooling fluids and is intended for the generation of data to enable registration of active substances. Samples containing the active substance under test are inoculated with defined suspensions of microorganisms and then incubated. The changes in the size of the populations over time are used to determine the basic antimicrobial properties of the active substances being evaluated. It is important to note that the results obtained may not describe the performance of the active substance under normal conditions of use.

This document describes a method for determining the basic efficacy of biocidal active substances in aqueous-based cooling fluids and is intended for the generation of data to enable registration of active substances. Samples containing the active substance under test are inoculated with defined suspensions of microorganisms as consortia and then incubated. The changes in the size of the populations over time are used to determine the basic antimicrobial properties of the active substances being evaluated. It is important to note that the results obtained may not describe the performance of the active substance under normal conditions of use.

This document describes a method to determine the resistance of paints and coatings to the growth of photosynthetic microorganisms and the effectiveness of biocides (preservatives) that might be used in them. Samples are inoculated with defined suspensions of microorganisms and then incubated. The changes in the size of the populations both over time and in treated compared with untreated materials are used to describe the basic antimicrobial properties of the materials. The results obtained may not describe the performance of the treated materials under normal conditions of use.

This document describes a method for determining the basic efficacy of biocidal active substances in aqueous based metalworking fluids and is intended for the generation of data to enable registration of active substances. Samples containing the active substance under test are repeatedly inoculated with defined suspensions of microorganisms and then incubated. The changes in the size of the populations over time are used to determine the basic antimicrobial properties of the active substances being evaluated. It is important to note that the results obtained may not describe the performance of the active substance under normal conditions of use. Version 1.04 Clarification of statistical methods, definition of growth and addition of Gram Positive bacterium to consortium.

This document describes a method for determining the basic efficacy of biocidal active substances for in-can preservation in aqueous based products and is intended for the generation of data to enable registration of active substances. Samples containing the active substance under test are repeatedly inoculated with defined suspensions of microorganisms and then incubated. The changes in the size of the populations over time are used to determine the basic antimicrobial properties of the active substances being evaluated. It is important to note that the results obtained may not describe the performance of the active substance under normal conditions of use.

Issue 2.0: Typographical corrections.

Issue 3.0: Correction of volumes stated in neutraliser validation method and revision of definition of minimum levels of growth.

Issue 4.0: The addition of a Precision Statement and typographical corrections.

Issue 5.0: Inclusion of a method to determine growth of the filamentous fungi and editing to align the method with other IBRG methods.

This document describes a method for determining the basic efficacy of biocidal active substances in polymer dispersions and is intended for the generation of data to enable registration of active substances.

Issue 2: Addition of Gram Positive bacterium to challenge consortium and harmonisation of statistical procedure with other methods.

Issue 3: Minor typographical corrections and update of Culture Collection numbers.

Issue 4: Changes to the inoculum (the addition of a filamentous fungi), inclusion of a method to determine growth of the filamentous fungi and editing to align the method with other IBRG methods.

This document describes a method for determining the basic efficacy of biocidal active substances in aqueous based paints and is intended for the generation of data to enable registration of active substances.

Issue 2: Addition of Gram Positive bacterium to challenge consortium and harmonisation of statistical procedure with other methods.

Issue 3: Minor typographical corrections and update of Culture Collection numbers.

Issue 4: Changes to the inoculum (the addition of a filamentous fungi), inclusion of a method to determine growth of the filamentous fungi and editing to align the method with other IBRG methods.